Journal: Nature Communications
Article Title: IL-1R signaling drives enteric glia-macrophage interactions in colorectal cancer
doi: 10.1038/s41467-024-50438-2
Figure Lengend Snippet: a Schematic representation of the murine AOM/DSS CRC model. Mice were intraperitoneally (i.p.) injected with azoxymethane (AOM, 10 mg/kg body weight) at day(d)0. Starting from d7, mice underwent 3 repetitive cycles of 1.5% dextran sodium sulfate (DSS) in drinking water as indicated. b GFAP Cre Ai14 fl/fl mice were subjected to the AOM/DSS model (Fig. 7a) using 1% DSS per cycle. Representative images of EPCAM (white), IBA1 (green) and tdTomato (magenta) in tumor sections at d70 (scale bar 100 µm) ( n = 3). c-e GFAP Wt IL-1R1 fl/fl and GFAP Cre IL-1R1 fl/fl mice were subjected to the AOM/DSS model (Fig. 7a). Tumors number and TAMs infiltration were assessed at d70. Tumor numbers of GFAP Wt IL-1R1 fl/fl and GFAP Cre IL-1R1 fl/fl littermates, representative images (left) and quantitative comparison of tumor numbers (right) ( n = 14 mice per genotype) ( c ). Corresponding absolute numbers of SPP1 + and C1Q + TAMs per mg tumor tissue ( n = 7 GFAP Wt IL-1R1 fl/fl , n = 9 GFAP Cre IL-1R1 fl/fl ) ( d ). Relative mRNA levels for Il6 , normalized to the housekeeping gene Rpl32 in naive ( n = 4 ) and AOM/DSS treated mice ( n = 7 GFAP Wt IL-1R1 fl/fl , n = 5 GFAP Cre IL-1R1 fl/fl ) ( e ). f GFAP Cre Ai14 fl/fl mice underwent the AOM/DSS model (Fig. 7a) using 1% DSS. Tumor or naive colon cells were isolated at d70 and FACS-sorted. Il6 expression levels of sorted tdTomato pos glial cells versus remaining tdTomato neg cells of naive and AOM/DSS-treated GFAP Cre Ai14 fl/fl mice ( n = 4). Expression displayed as fold to Rpl32 and relative to naive tdTomato neg cells. g SOX10 CreERT2 Ai14 fl/fl mice were i.p. injected with Tamoxifen (1 mg in 100 µL sterile corn oil) on d–7, −6, and −5. Subsequently, mice were subjected to the AOM/DSS model (Fig. 7a) using 2% DSS per cycle. Representative image of tdTomato (magenta), IL-6 (green), and DAPI (blue) in tumor section at d70 (scale bar 100 µm) ( n = 3). Data are represented as mean ± SEM ( c – f ). Statistical analysis: unpaired two-tailed t-test ( c ), unpaired two-tailed Mann-Whitney test ( d ), and two-way ANOVA with correction for multiple comparisons ( e , f ). * p < 0.05, *** p < 0.0005, ns not significant. Source data and exact p values are provided as a file.
Article Snippet: Female Sox10 CreERT2 Ai14 fl/fl mice were i.p. injected with Tamoxifen (MP biomedicals, 1 mg in 100 µL sterile corn oil) on days −7, −6, and −5.
Techniques: Injection, Comparison, Isolation, Expressing, Sterility, Two Tailed Test, MANN-WHITNEY
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